The CityPilot will celebrate its world public premiere in the Mercedes-Benz Future Bus technology platform, on Airport Line 300 in the Netherlands. This links the Dutch metropolis of Amsterdam with Amsterdam's Schiphol airport and the town of Haarlem. The route has a total length of 37.8 km, making it Western Europe's longest BRT line.

For the world premiere of the CityPilot, Mercedes-Benz is using the 19 km stretch from Amsterdam-Schiphol airport to Haarlem. This has eleven stops, with a driving time of around 30 minutes. Depending on the time of day, the cycle time on this line is six to ten minutes. On average, Airport Line 300 is used by over 125 000 passengers each day, and it is operated by the Dutch transport company Connexxion.

The route profile of Airport Line 300 is demanding: the bends are sometimes very tight, and the oncoming traffic lane is not physically separated. The route also has 22 traffic lights, three tunnels and includes high speeds of up to 70 km/h. The bus stops are raised, allowing passengers to enter the buses conveniently at ground level.